Figure 9.

Increased Bcl-2 expression in the cellular compartments of cystic fibrosis (CF) cells. Nuclear, endoplasmic reticulum (ER), and mitochondrial fractions were prepared from (1) 16HBEo-, (2) CF41o-, and (3) CF45o- cells. Western blots were performed using antibodies against Bcl-2, cytochrome c oxidase (mitochondrial marker), protein disulphide isomerase (PDI, ER-specific protein) and lamin C (nuclear marker) (A). (B) Bcl-2 expression in the ER fraction of 16HBEo- cell line stably transfected with sense (S) and antisense (AS) CFTR oligonucleotide. (C) Total Bcl-2 content in cellular lysates of control and adenovirally transduced primary human bronchial epithelial cells grown on collagen-coated culture dishes. The bars represent means of data of two individual experiments (n = 4). *Significant difference (P < 0.05) from control.