TABLE 2.
SERCA2 KNOCKDOWN ENHANCES CELL DEATH
| Control siRNA
|
SERCA2 siRNA
|
|||
|---|---|---|---|---|
| Treatment | Apoptosis (%) | Necrosis (%) | Apoptosis (%) | Necrosis (%) |
| TNF + IL-1β (10 ng/ml for 18 h) | 17.53 ± 1.83 | 8.54 ± 0.55 | 29.46 ± 0.72* | 9.53 ± 0.39 |
| H2O2 (500 μM for 18 h) | 9.66 ± 0.33 | 14.9 ± 0.85 | 14.00 ± 1.00* | 28.33 ± 1.66* |
Definition of abbreviation: SERCA2 = sarcoendoplasmic reticulum Ca2+-ATPase; TNF = tumor necrosis factor.
*
Significant difference from control siRNA-treated cells (P < 0.05). n = 3, experiment repeated three times.