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. 2009 Apr 24;65(Pt 5):518–521. doi: 10.1107/S1744309109013670

Figure 1.

Figure 1

Purification profile of β-MSP from human seminal plasma. (a) Purification of β-MSP on an anion-exchange column (DEAE-Sephacel). The graph was plotted of elution volume in millilitres (x axis) against absorbance (y axis) at 280 nm. Fraction 2 (indicated by a bar) was collected and concentrated. (b) The fraction from the previous step was loaded onto a HiLoad Superdex 75 (16/60) column. The fraction containing β-MSP was pooled and concentrated for crystallization. Inset: SDS–PAGE of purified β-MSP from human seminal plasma under reducing conditions. Lane M, molecular-weight markers (kDa); lane 1, fraction 2 (indicated by a bar) from the DEAE column; lane 2, purified β-MSP from the Superdex 75 column; lane 3, β-MSP band from a crystal dissolved in acetonitrile and washed with crystallization buffer.