Table 3.
Ada2p promoter occupancies of glycolysis and pyruvate metabolism genes in the wt and gal4 mutant
| Orf19 | Gene name | Description | Gal4p binding motif | Position | Binding ratio |
|
|---|---|---|---|---|---|---|
| wt | gal4 | |||||
| Glycolysis | ||||||
| orf19.4941 | TYE7 | bHLH transcription factor | GCC (N11) GGC | −1214 | 2.11 | 0.91 |
| orf19.3575 | CDC19 | Putative pyruvate kinase | CGG (N11) TCG | −263 | 2.14 | 0.91 |
| orf19.3967 | PFK1 | Alpha subunit of phosphofructokinase | TCC (N11) GGG | −753 | 2.21 | 0.95 |
| orf19.6540 | PFK2 | Beta subunit of phosphofructokinase | CCC (N11) GGC | −586 | 2.01 | 1.00 |
| orf19.3997 | ADH1 | Alcohol dehydrogenase | CGG (N11) CCG | −613 | 2.04 | 1,03 |
| orf19.3888 | PGI1 | Glucose-6-phosphate isomerase | TCC (N11) GGC | −508 | 1.84 | 0.96 |
| orf19.6745 | TPI1 | Triose-phosphate isomerase | CGT (N11) CCG | -291 | 1.79 | 0.84 |
| orf19.4617 | FBA1 | FBA1 Putative fructose-bisphosphate aldolase | GCC (N11) GGT | −397 | 1.78 | 1.1 |
| orf19.5338 | GAL4 | Transcription factor with zinc cluster DNA-binding motif | CGA (N11) CCG | −710 | 1.5 | 1.04 |
| orf19.1067 | GPM2 | Phosphoglycerate mutase | CGA (N11)CCG | −268 | 1.55 | 1.15 |
| orf19.6814 | TDH3 | Glyceraldehyde-3-phosphate dehydrogenase | ACC (N11) GGC | −303 | 1.65 | 1.08 |
| orf19.395 | ENO1 | ENO1 Enolase (2-phospho-D-glycerate-hydrolyase) | GCC (N11) GGT | −308 | 2.6 | 1.03 |
| orf19.903 | GPM1 | Phosphoglycerate mutase | AGG (N11) CCG | −268 | 2.55 | 1.13 |
| orf19.3651 | PGK1 | Phosphoglycerate kinase | ACC (N11) GGC | −456 | 2.5 | 0.98 |
| Pyruvate metabolism | ||||||
| orf19.2877 | PDC11 | Pyruvate decarboxylase | GCC (N11) GGG | −324 | 1.95 | 0.89 |
| orf19.5021 | PDX1 | Dihydrolipoamide dehydrogenase (E3)-binding protein of the mitochondrial pyruvate dehydrogenase (PDH) complex | GCC (N11) GGT | −164 | 2.02 | 1.15 |
| orf19.3097 | PDA1 | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex | GCC (N11) GGT | −141 | 1.86 | 0.87 |
| orf19.5294 | PDB1 | E1 beta subunit of the pyruvate dehydrogenase (PDH) complex | GCC (N11) GGT | −196 | 1.87 | 0.92 |
| orf19.6561 | LAT1 | Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex | GCC (N11) GGT | −242 | 1.8 | 0.93 |
| Translation and ribosome assembly | ||||||
| orf19.2935 | RPL10 | Cytoplasmic ribosomal subunits | — | — | 1.72 | 1.71 |
| orf19.6265 | RPS22A | Cytoplasmic ribosomal suunits | — | — | 1.56 | 2.15 |
Occupancy of RPL10 and RPS22Apromoters is shown as a control. Detection of Gal4p motif was performed as described by Hogues et al. (2008).