Table 2.

prm3 is a unilateral karyogamy mutant

Cross	% WT
WT × WT	94
WT × prm3Δ	91
prm3Δ × prm3Δ	6
prm3Δ [PRM3] × prm3Δ	72
prm3Δ [GFP-PRM3] × prm3Δ	68
prm3Δ [FLAG-PRM3] × prm3Δ	73

Matings were performed at 30°C for 2.5 h on nitrocellulose filters on YEPD plates, fixed in 3:1 methanol:acetic acid, and stained with DAPI to visualize the nuclei. Zygotes with one nucleus were counted as Kar⁺, whereas zygotes with two nuclei were counted as Kar⁻. The percent WT number is the percentage of zygotes that are Kar⁺ over total. Numbers represent the average of at least two independent experiments, with ∼100 zygotes counted in each experiment. The strains used were prm3 (MS7590 × MS7591) and WT (MS7592 × MS7593), and the plasmids were MR5062, MR5063, and MR5065.