Table 4.
PRM3 and KAR5 interact genetically
| Cross | % WT | % Kar− |
|---|---|---|
| WT × prm3Δ | 97 | 3 |
| WT × kar5Δ | 89 | 11 |
| WT × kar5-1162 | 67 | 33 |
| prm3Δ × prm3Δ | 6 | 94 |
| kar5Δ × kar5Δ | 6 | 94 |
| kar5-1162 × kar5-1162 | 4 | 96 |
| prm3Δ × kar5Δ | 72 | 28 |
| prm3Δ × kar5-1162 | 28 | 72 |
| prm3Δ × prm3Δ | 3 | 97 |
| prm3Δ [2μ PRM3] × prm3Δ | 53 | 47 |
| prm3Δ [2μ PRM3] × prm3Δ [2u PRM3] | 68 | 32 |
| prm3Δ [2μ KAR5] × prm3Δ | 17 | 83 |
| prm3Δ [2μ KAR5] × prm3Δ [2u KAR5] | 32 | 68 |
Matings were performed at 30°C for 2.5 h on nitrocellulose filters on YEPD plates, fixed in 3:1 methanol:acetic acid, and stained with DAPI to visualize the nuclei. Zygotes with one nucleus were counted as WT, whereas zygotes with two nuclei were counted as Kar−. Numbers represent the average of at least two independent experiments, with ∼100 zygotes counted in each experiment. The strains used were: prm3 (MS7590 × MS7591), kar5 (MS7673 × MS7674), and kar5-1162 (MS7666 × MS7667). Plasmids used were pMR5757 and pMR5760.