Figure 4.

ADAM-17 is involved in Met shedding. MCF10A cells were transfected or not with siRNA control or targeting the ADAM-17. Cells were then treated or not overnight with 1 μM E compound and the following day 5 h with 10 μM lactacystin. Proteins were resolved by 10% SDS-PAGE and analyzed by Western blotting with antibodies directed against the kinase domain of Met (WB Met). The filter was stripped and reprobed with an antibody directed against the ADAM-17 and against ERK2 to assess the loading. The two parts of the panels were on the same gel. Arrows indicate the position of Met-CTF.