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. 2009 May 1;20(9):2495–2507. doi: 10.1091/mbc.E08-09-0969

Figure 5.

Figure 5.

HGF/SF does not influence PS-RIP of Met. (A) MDCK cells were treated or not overnight with 1 μM E compound and the following day 10 h with ALLN. Cells were then stimulated with HGF/SF (30 ng/ml) at the indicated time. (B) MDCK cells transiently transfected with either the empty vector or the vector expressing full-length human wild-type Met (Met WT) or kinase-dead Met (Met KD) were treated or not overnight with 1 μM E compound. (A and B) Proteins were resolved by 10% SDS-PAGE and analyzed by Western blotting with antibodies directed against the kinase domain of Met (WB Met). The filter was stripped and reprobed with an antibody directed against the phosphorylated tyrosine residues of the kinase domain of Met. Arrows indicate the positions of Met, Met-CTF and phosphorylated Met and Met-CTF.