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. 2009 May 1;20(9):2495–2507. doi: 10.1091/mbc.E08-09-0969

Figure 7.

Figure 7.

PS-RIP occurs on the mature TRK-Met chimera at the membrane. (A) MDCK cells stably expressing TRK-Met-juxta or TRK-Met were treated or not overnight with 25 μM GM6001. Nuclei were detected by Hoechst staining (Hoechst, blue staining), and immunofluorescence staining was done with an anti-mouse Met antibody (Anti-mouse Met, red staining). Magnification, ×100. (B) TRK-Met– and TRK-Met-juxta–expressing cells were stained (red, open histogram) or not (black, filled histogram) with a phycoerythrin-coupled antibody directed against the extracellular domain of TRKA, and fluorescence was analyzed by flow cytometry. (C) TRK-Met– and TRK-Met-juxta–expressing cells were treated or not overnight with 2.5 μM SU11274. Immunoprecipitation was performed with an anti-mouse Met antibody. Proteins were resolved by 10% SDS-PAGE and analyzed by Western blotting with an antibody against phosphorylated tyrosine residues of the kinase domain of Met. The filter was stripped and reprobed with an antibody directed against mouse Met. Arrows indicate the positions of the mature and immature forms of TRK-Met and TRK-Met-juxta.