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. 2009 May 1;20(9):2495–2507. doi: 10.1091/mbc.E08-09-0969

Figure 8.

Figure 8.

PS-RIP of TRK-Met chimera prevents its basal activation and the induction of invasive growth. (A) Scattering from cell islets. MDCK stably expressing TRK-Met and TRK-Met-juxta were seeded at low density. The next day the cells were cultured in the presence or absence of 2.5 μM SU11274 and/or 100 ng/ml NGF. Magnification, ×40 (B) MDCK stably expressing TRK-Met and TRK-Met-juxta were seeded at low density. The next day, the cells were fixed and stained for F-actin with phalloidin. Magnification, ×100. (C) Morphogenesis on Matrigel gels. MDCK stably expressing TRK-Met and TRK-Met-juxta were cultured on Matrigel gels. The following day, cells were incubated with or without SU11274 and/or NGF. Magnification, ×60. (D) MDCK stably expressing TRK-Met and TRK-Met-juxta, cultured in invasion chambers, were treated or not with 5 μM SU11274, a Met kinase inhibitor. The relative number of infiltrated cells was determined after staining of nuclei with Hoechst (n = 3, ± SD).