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Analysis of stability of the VEEV TRD- and VEEV TC-83-specific, 5′-terminal stem-loops by NMR. The 31-nt-long RNA molecules, representing nt 1–31 of the VEEV TRD and TC-83 genomes, were synthesized in vitro and prepared for NMR as described in Materials and Methods. The imino regions of the 1D NOESY spectra presented here were collected at 5° C, 15° C, and 25° C. The signals were assigned from the 2D NOESY spectra, using sequential NOE connectivities.
