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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Osteoarthritis Cartilage. 2009 Mar 5;17(4):423–426. doi: 10.1016/j.joca.2009.02.005

Fig. 2.

Fig. 2

Direct confirmation by tandem mass spectrometry that the α1(II) collagen sequence from human cartilage at helix residues 631-636 is PAGF not TSGI. The upper panel shows the tryptic peptide profile by total ion current (TIC) on HPLC from CNBr-fragment α1(II)CB10. The middle panel shows the mass spectrum from the peptide encompassing the Helix-II epitope of mass that fits the internal sequence PAGF (not TSGI) and the lower panel the MS/MS fragmentation spectrum of the 12675+ ion confirming the structure shown.