Table I.
Primers and probes used in Taqman assays to determine polymorphic gene frequencies
| Custom Taqman SNP genotyping assays | |||
| Gene | Polymorphism | Primer | Probe |
| IL-6 | −174G/C | FP: 5′-GACGACCTAAGCTGCACTTTTC | 5′-VICCGGAAGAAAACATTTMGBNFQ |
| RP: 3′-GGGCTGATTGGAAACCTTATTAAGATTG | 5′-FAMCGGAAGAAAAGATTTMGBNFQ | ||
| IL-12 | 1188A/C | FP: 5′-GGATCACAATGATATCTTTGCTGTATTT | 5′-VICCATTTAGCATCTAACTATACMGBNFQ |
| RP: 3′-TGAGAGCTGGAAAATCTATACATAAATTAGC | 3′-FAMTTTAGCATCGAACTATACMGBNFQ | ||
| IL-18 | −137G/C | FP: 5′-GGCACAGAGCCCCAACTTT | 3′-VICCGGAAGAAAACATTTMGBNFQ |
| RP: 3′-GCAGAGGATACGAGTACTTCTTTTAATG | 3′-FAMCGGAAGAAAAGATTTMGBNFQ | ||
| ABI predesigned SNP genotyping assays | |||
| Gene | Polymorphism | dbSNP ID | ABI assay ID |
| IL-2 | −330T/G | rs2069762 | C_15859930_10 |
| IL-4 | −589C/T | rs2243250 | C_16176216_10 |
| IL-10 | −1082G/A | rs1800896 | C_1747360_10 |
| TNFα | −308G/A | rs1800629 | C_7514879_10 |
| IFNγ1 | IVS3 +284G/A | rs1861494 | C_2683477_10 |
| IFNγ2 | −764C/G | rs2069707 | C_15859751_10 |
FP, forward primer; RP, reverse primer.