Skip to main content
. 2009 Feb 23;30(5):861–868. doi: 10.1093/carcin/bgp050

Fig. 1.

Fig. 1.

Proepithelin mRNA is overexpressed in bladder cancer and is required for cell growth of T24 transitional cell carcinoma-derived cells in the absence of serum. (A) Expression array analysis of multiple bladder cancer microarray data sets was analyzed and statistical significance was calculated. Classes A and B proepithelin expression in normal tissues and bladder cancer; in blue is indicated the expression in normal tissues, whereas in red the expression in bladder cancers. (A) Sanchez-Carbayo et al. (24) (t-test = −7.768; P = 6.3 × 10−12; 52 normal urothelium, 105 bladder tumors: 33 superficial and 72 invasive); (B) Dyrskjot et al. (25) (t-test = −4.98; P = 1.6 × 10−5; 14 normal urothelium, 40 bladder tumors: 27 superficial and 13 invasive). Classes C, D and E: proepithelin expression associated to pathologic and prognostic parameters. (C) Blaveri et al. (26) (t-test = −3.41; P = 0.004); in blue is indicated the expression in low-grade bladder cancers, whereas in red the expression in high-grade cancers. (D) Blaveri et al. (26) (t-test = −2.405; P = 0.025); in blue is indicated the expression in patients alive after 5 years of follow-up after treatment, whereas in red the expression in patients dead in the 5 years of follow-up. (E) Lindgren et al. (27) (t-test = −2.057; P = 0.05); in blue is indicated the expression in Ta stage cancers, whereas in red the expression in T1 stage cancers. (B) The generation of T24/pRS and T24/shPEP has been described in Materials and Methods. Proepithelin expression in lysates and CM (medium) (40 μg) of parental (P), vector-transfected (C) and shRNA proepithelin T24 cells (shPEP) was detected by immunoblot. (C) Cell growth in SFM was determined as described in Materials and Methods. The experiment is the average of four independent experiments run in duplicates ± standard deviation. *P < 0.05 compared with vector-transfected control cells (second and fifth columns). At 48 h, SFM of proepithelin-depleted cells was also supplemented with 400 ng/ml of recombinant proepithelin (shPEP + PEP). ♦P < 0.05 compared with proepithelin-depleted T24 cells in SFM.