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. 2009 Feb 23;30(5):861–868. doi: 10.1093/carcin/bgp050

Fig. 2.

Fig. 2.

Endogenous proepithelin promotes in vitro closure of a wound and invasion of T24 cells. (A) The in vitro wound healing motility assay in T24 cells in SFM was performed as described in Materials and Methods. Cells were analyzed with a cell live microscope using the Metamorph Image Acquisition and Analysis software (Universal Imaging) (×100). Ten fields per plate were examined. (B) For migration assay, T24 cells were seeded on FluoroBloks and allowed to migrate for 24 h. Top, representative fields of migrated T24 cells (magnification, ×100); bottom, quantification of migrated T24 cells. The data are the average of three independent experiments run in duplicates ± standard deviation. (C) Quantification of invading T24 cells plated on Matrigel-coated FluoroBloks and allowed to invade for 24 h. Data are expressed as invading cells per field. Mean ± SD of three independent experiments done in duplicate. *P < 0.01 versus T24 cells; ♦P < 0.01 versus vector-transfected T24 cells.