Fig. 3.

Endogenous proepithelin stimulates the ability of T24 cells to evade from Matrigel drops. (A) T24, T24/pRS and T24/shPEP cells were included in a Matrigel drop and allowed to evade for 2, 3 and 5 days. The data are the average of three independent experiments in quintuplicate (±standard deviation). *, **, ***P < 0.05 compared with vector-transfected control cells. (B) A typical image from three independent experiments performed in quintuplicate is shown. The dotted line indicates the edge of Matrigel drops. (C) Quantification of the migration distance covered by the various cell lines over a 5 days period. The distance covered was calculated by converting pixel to millimeters. The data are the average of three independent experiments in quintuplicate (±SD). *P < 0.05 compared with vector-transfected control cells.