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. 2009 Feb 23;30(5):861–868. doi: 10.1093/carcin/bgp050

Fig. 4.

Fig. 4.

Proepithelin depletion severely reduces the activation of the Akt and MAPK pathways. (A) CM from T24, T24/pRS and T24/shPEP cells was prepared as described (22). T24 cells were serum starved for 24 h and then stimulated with the CM for 10 and 30 min. The activation of p90RSK, Akt, ERK1/2 and S6 ribosomal protein was analyzed by western immunoblot using the PathScan Multiplex Western Cocktail I (Cell Signaling Technology). ElF4E protein is the control to monitor the loading of the samples. Blot is representative of three independent experiments. (B) Recombinant proepithelin restores Akt and ERK1/2 activation in T24/shPEP. T24/shPEP cells were serum starved for 24 h and then stimulated with 400 ng/ml of recombinant proepithelin for 10 and 30 min. The activation of Akt and ERK1/2 was analyzed by western immunoblot using the PathScan Multiplex Western Cocktail I (Cell Signaling Technology). Blot is representative of two independent experiments.