Fig. 2.

Klenow exo minus fragment is able to incorporate CldCTP into the newly synthesized strand as well as synthesize past ClC in the template strand. (a) Comparable polymerase incorporation of dCTP and CldCTP into the newly synthesized strand. Bottom band in each lane represents the ³²P-labeled 22mer primer. Top band in represents the 30mer full-length extension product. (b) Comparable polymerase extension past C and ClC in template strand. Bottom most band in each lane represents the ³²P-labeled 23mer primer. Top most band represents the 30mer full-length extension product.