Fig. 1. Mn²⁺ uptake by B. japonicum.

A. ⁵⁴Mn²⁺ uptake was measured in parent strain USDA110 in cells grown in media containing 1 μM (closed circles) or 50 μM MnCl₂ (open circles). At time zero, 5 nM ⁵⁴Mn²⁺ was added to the cells in assay medium, and aliquots were subsequently taken at various time points and counted. B. Competition of ⁵⁴Mn²⁺ uptake with unlabeled Mn²⁺ or Fe²⁺. Cells of the parent strain were grown in media containing no added manganese to induce Mn²⁺ transport activity. The assay medium containing cells was supplemented with either no metal (closed circles) or with 1 μM MnCl₂ (closed squares) or 1 μM FeSO₄ (open squares) immediately prior to addition of 5 nM ⁵⁴Mn.