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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Microbiol. 2009 Mar 4;72(2):399–409. doi: 10.1111/j.1365-2958.2009.06650.x

Fig. 4. Dependence on manganese or iron supplementation for growth of B. japonicum parent or mntH strains.

Fig. 4

Growth media were inoculated with 5 × 105 cells/ml of parent strain (closed circles) or the mntH mutant (open circles) and grown in media containing (A) 50 μM MnCl2 and 20 μM FeCl3, (B) no exogenous manganese and 20 μM FeCl3, (C) 50 μM MnCl2 and no exogenous iron, or (D) no exogenous manganese and no exogenous iron. Unsupplemented media contains 0.2 μM and 0.3 μM manganese and iron, respectively. Aliquots were taken at the indicated time points and the optical density was measured at 540 nm (OD540).