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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Microbiol. 2009 Mar 4;72(2):399–409. doi: 10.1111/j.1365-2958.2009.06650.x

Fig. 8. Effect of manganese on B. japonicum Fur binding to the mntH promoter.

Fig. 8

EMSA was carried out using 100 pM 32P-labelled mntH promoter DNA titrated with various concentrations of Fur in the presence (closed squares) or absence (open squares) of 100 μM MnCl2. Bound and unbound DNA was resolved on a 5% non-denaturing polyacrylamide gel and visualized by autoradiography. Autoradiograms were scanned, and bands were quantified to determine bound and unbound DNA.