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. 2009 Mar 13;94(5):720–724. doi: 10.3324/haematol.2008.001784

Figure 1.

Figure 1.

The heterozygous -153C/T mutation decreases hepcidin promoter transcription activity. HepG2 cells were transfected with the -1024/BMP-RE wt or -1024/BMP-RE mut Hep/Luc constructs and were treated with BMP9 (lanes 4,5), BMP4 (lanes 6,7), IL-6 (lanes 8,9,10) or not (lanes 1–2) for 48 h. Luciferase activity values represent Firefly/Renilla Luciferase activity ratios relative to that obtained with the -1024/BMP-RE wt Hep/Luc plasmid construct, which was arbitrarily set at 100%. Data represent means of at least three independent experiments (± SEM). *p<0.001 compared to the -1024/BMP-RE wt Hep/Luc construct and #p<0.001 compared to the -1024/BMP-RE mut determined by 1-way ANOVA and the Student-Newman-Keuls comparisons test.