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Whole-mount immunolocalization of Cnox-2 in the gastrozooid. Immunoreactive nuclei are stained blue. Larger, irregular blue shapes are nonspecific staining. (a) Lateral view of a gastrozooid. (b and d) Magnification of representative regions of a hypostome and body column. (c and e) 4′,6-diamidino-2-phenylindole (DAPI)-stained image of identical regions, showing total nuclei. (Bars = 0.1 mm.)
