Table 1.

Family A polymerase-mediated incorporation (k_cat/K_M, M^-1min^-1) of dXTP opposite unnatural nucleotides in the template.^a

5′—dTAATACGACTCACTATAGGGAGA
3′—dATTATGCTGAGTGATATCCCTCT(Y)GCTAGGTTACGGCAGGATCGC
X	Y	Kfb	T7	Taq
dA	dT	(3.2 ± 0.6) × 108	(1.5 ± 0.4) × 107	(4.0 ± 0.5) × 107
dG	dT	(5.7 ± 0.5) × 104	(9.8 ± 0.7) × 102	(2.5 ± 0.5) × 104
dPICS	dPICS	(2.4 ± 0.3) × 105	<1.0 × 103	(2.6 ± 0.5) × 104
dA	dPICS	(1.8 ± 0.4) × 103	<1.0 × 103	<1.0 × 103
dC	dPICS	(1.8 ± 0.5) × 103	<1.0 × 103	<1.0 × 103
dG	dPICS	(1.7 ± 0.4) × 103	<1.0 × 103	<1.0 × 103
dT	dPICS	(1.2 ± 0.2) × 104	<1.0 × 103	(1.2 ± 0.3) × 103
d7AI	d7AI	(2.2 ± 0.5) × 105	(2.2 ± 0.3) × 103	(1.2 ± 0.1) × 104
dA	d7AI	(5.9 ± 1.0) × 103	(1.2 ± 0.5) × 103	(4.1 ± 0.1) × 103
dC	d7AI	(5.5 ± 0.9) × 103	(1.1 ± 0.4) × 103	<1.0 × 103
dG	d7AI	(1.6 ± 0.4) × 103	<1.0 × 103	<1.0 × 103
dT	d7AI	(1.6 ± 0.6) × 103	<1.0 × 103	(2.3 ± 0.6) × 103
d3FB	d3FB	(2.1 ± 0.8) × 106	(5.3 ± 1.6) × 104	(1.5 ± 0.1) × 104
dA	d3FB	(5.4 ± 1.0) × 105	(1.1 ± 0.1) × 105	(9.1 ± 2.0) × 104
dC	d3FB	(2.1 ± 1.2) × 103	(1.8 ± 0.2) × 103	(2.1 ± 2.9) × 103
dG	d3FB	(1.8 ± 0.6) × 103	<1.0 × 103	<1.0 × 103
dT	d3FB	(9.5 ± 1.1) × 104	(8.1 ± 0.3) × 104	(2.8 ± 0.7) × 104
dMMO2	d5SICS	(3.6 ± 0.7) × 105	(3.7 ± 0.1) × 104	(8.6 ± 0.9) × 104
d5SICS	d5SICS	(2.7 ± 0.9) × 104	<1.0 × 103	(3.2 ± 0.7) × 103
dA	d5SICS	(2.2 ± 0.3) × 104	(1.7 ± 0.2) × 103	(2.7 ± 0.7) × 103
dC	d5SICS	<1.0 × 103	<1.0 × 103	<1.0 × 103
dG	d5SICS	(1.3 ± 0.8) × 105	<1.0 × 103	(3.7 ± 0.5) × 104
dT	d5SICS	(1.3 ± 0.4) × 104	<1.0 × 103	(3.7 ± 0.9) × 103
d5SICS	dMMO2	(4.7 ± 0.4) × 107	(1.1 ± 0.2) × 106	(3.5 ± 0.6) × 106
dMMO2	dMMO2	(1.2 ± 0.1) × 105	(1.5 ± 0.3) × 104	(2.1 ± 0.6) × 104
dA	dMMO2	(1.0 ± 0.1) × 105	(5.8 ± 1.7) × 104	(1.1 ± 0.3) × 105
dC	dMMO2	<1.0 × 103	<1.0 × 103	<1.0 × 103
dG	dMMO2	<1.0 × 103	<1.0 × 103	<1.0 × 103
dT	dMMO2	<1.0 × 103	(1.9 ± 0.1) × 103	(8.2 ± 0.8) × 103

^aSee text and Supporting Information for experimental details.

^bRef. ¹¹-¹⁴. Error reported is standard deviation calculated from three independent experiments.