Table 2.

Family A polymerase-mediated extension (k_cat/K_M, M^-1min^-1) of dX:dY pairs.^a

5′—dTAATACGACTCACTATAGGGAGA(X)
3′—dATTATGCTGAGTGATATCCCTCT(Y)GCTAGGTTACGGCAGGATCGC
X	Y	Kfb	T7	Taq
dA	dT	(1.7 ± 0.2) × 108	(1.3 ± 0.3) × 107	(1.9 ± 0.2) × 107
dG	dT	(4.8 ± 0.5) × 105	(1.8 ± 0.1) × 104	(7.4 ± 1.2) × 103
dPICS	dPICS	<1.0 × 103	(2.8 ± 0.4) × 103	<1.0 × 103
dA	dPICS	nd	<1.0 × 103	nd
dT	dPICS	(2.0 ± 1.1) × 105	nd	(1.6 ± 0.1) × 103
d7AI	d7AI	<1.0 × 103	<1.0 × 103	<1.0 × 103
dA	d7AI	(1.9 ± 0.2) × 103	<1.0 × 103	<1.0 × 103
dC	d7AI	(4.5 ± 0.7) × 103	<1.0 × 103	nd
d3FB	d3FB	(3.3 ± 0.6) × 105	(1.5 ± 0.4) × 105	(1.6 ± 0.4) × 104
dA	d3FB	(6.3 ± 1.7) × 104	<1.0 × 103	<1.0 × 103
dC	d3FB	(1.4 ± 0.5) × 104	nd	nd
dG	d3FB	<1.0 × 103	nd	nd
dT	d3FB	(1.9 ± 0.4) × 105	(3.4 ± 0.1) × 103	(1.1 ± 0.2) × 103
dMMO2	d5SICS	(1.9 ± 0.2) × 106	(5.4 ± 1.2) × 105	(2.2 ± 0.2) × 104
d5SICS	d5SICS	<1.0 × 103	<1.0 × 103	<1.0 × 103
dA	d5SICS	nd	<1.0 × 103	<1.0 × 103
dG	d5SICS	(4.9 ± 0.5) × 103	<1.0 × 103	<1.0 × 103
dT	d5SICS	(4.0 ± 0.2) × 105	nd	(4.7 ± 0.7) × 103
d5SICS	dMMO2	(6.7 ± 1.1) × 105	(1.1 ± 0.2) × 106	(6.4 ± 1.6) × 104
dMMO2	dMMO2	(5.3 ± 0.9) × 103	(5.9 ± 0.4) × 103	<1.0 × 103
dA	dMMO2	(4.6 ± 0.2) × 104	<1.0 × 103	(1.7 ± 0.1) × 103
dT	dMMO2	nd	nd	(1.1 ± 0.1) × 104

^aSee text and Supporting Information for experimental details.

^bRef. ¹¹-¹⁴. Error reported is standard deviation calculated from three independent experiments. nd, not determined.