Table 5.

Family B and family X polymerase-mediated extension (k_cat/K_M, M^-1min^-1) of dX:dY pairs.^a

5′—dTAATACGACTCACTATAGGGAGA(X)
3′—dATTATGCTGAGTGATATCCCTCT(Y)GCTAGGTTACGGCAGGATCGC
X	Y	Vent	Therminator	Pol β
dA	dT	(2.9 ± 0.6) × 106	(3.4 ± 1.1) × 106	(2.1 ± 0.2) × 105
dG	dT	(1.5 ± 0.1) × 104	(7.1 ± 1.6) × 105	(2.8 ± 0.4) × 103
dPICS	dPICS	<1.0 × 103	(1.1 ± 0.2) × 104	(9.7 ± 1.0) × 103
dA	dPICS	<1.0 × 103	(1.4 ± 0.1) × 104	<1.0 × 103
dG	dPICS	<1.0 × 103	nd	nd
dT	dPICS	(1.1 ± 0.1) × 103	nd	(1.3 ± 0.1) × 103
d7AI	d7AI	<1.0 × 103	(1.2 ± 0.1) × 105	(4.4 ± 0.5) × 104
dA	d7AI	<1.0 × 103	(8.7 ± 1.7) × 103	(7.6 ± 0.3) × 103
dT	d7AI	(1.0 ± 0.1) × 103	(2.8 ± 0.4) × 104	nd
d3FB	d3FB	(5.7 ± 0.2) × 103	(7.8 ± 0.8) × 105	(8.3 ± 0.5) × 104
dA	d3FB	(1.1 ± 0.4) × 103	(3.0 ± 0.4) × 103	(6.7 ± 0.2) × 103
dT	d3FB	<1.0 × 103	nd	nd
dMMO2	d5SICS	(6.2 ± 1.6) × 103	(4.6 ± 0.3) × 105	(4.4 ± 0.5) × 104
d5SICS	d5SICS	<1.0 × 103	(4.5 ± 1.1) × 103	(2.2 ± 1.0) × 104
dA	d5SICS	<1.0 × 103	(8.8 ± 1.5) × 104	(3.9 ± 0.3) × 103
dC	d5SICS	nd	nd	(5.3 ± 0.2) × 103
dG	d5SICS	<1.0 × 103	(1.5 ± 0.2) × 105	(4.1 ± 0.1) × 103
dT	d5SICS	<1.0 × 103	(2.3 ± 0.5) × 105	(4.4 ± 0.1) × 103
d5SICS	dMMO2	(7.9 ± 0.4) × 103	(2.5 ± 0.6) × 105	(4.0 ± 2.1) × 105
dMMO2	dMMO2	(1.2 ± 0.1) × 103	(6.8 ± 2.0) × 104	(1.2 ± 0.4) × 104
dA	dMMO2	nd	(1.5 ± 0.1) × 103	(2.2 ± 0.1) × 104
dC	dMMO2	nd	(3.5 ± 0.2) × 104	(2.1 ± 0.3) × 104
dG	dMMO2	nd	(1.2 ± 0.1) × 103	nd
dT	dMMO2	(3.8 ± 0.8) × 103	(4.4 ± 1.9) × 104	nd

^aSee text and Supporting Information for experimental details. Error reported is standard deviation calculated from three independent experiments. nd, not determined.