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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: Exp Physiol. 2008 Mar 20;93(5):613–621. doi: 10.1113/expphysiol.2007.040246

Figure 3. Metabolism of Ang II by cardiac membranes.

Figure 3

Angiotensin II was incubated with cardiac membranes (50 μg) for 120 min at 37°C and the metabolites separated by HPLC. A, Ang II was hydrolysed primarily to Ang(1−7) in the heart membranes from wild-type mice. B, increased metabolism of Ang II to Ang(1−7) in the heart membranes of ACE−/− mice. C, addition of the ACE2 inhibitor MLN4760 (10 μm) reduced the peak of Ang(1−7) and increased the peak of Ang II in ACE−/− mice. D, attenuated metabolism of Ang II in the in the heart membranes of ACE2−/− mice. The HPLC metabolism results are representative of four separate experiments.