Table I.
Endogenous lipid pool sizes and FA composition
DAG, PC, and TAG amounts present in 13 to 14 mg dry weight developing embryos precultured for 3 d, as measured by ESI-MS/MS. The FA composition is calculated from the molecular species in Figure 1. FA values are represented as mol % among each lipid class.
| DAG | PC | TAG | |
|---|---|---|---|
| nmoles lipid embryo−1 | 104.1 ± 25.3 | 270.5 ± 27.8 | 4,658.8 ± 179.0 |
| % mol FA | |||
| 16:0 | 14.4 ± 0.4 | 17.2 ± 0.4 | 14.9 ± 0.3 |
| 18:0 | 8.2 ± 0.4 | 9.4 ± 0.3 | 4.6 ± 0.05 |
| 18:1 | 30.6 ± 0.3 | 28.6 ± 0.2 | 26.4 ± 0.2 |
| 18:2 | 36.9 ± 0.3 | 35.0 ± 0.5 | 41.2 ± 0.2 |
| 18:3 | 9.9 ± 0.3 | 9.9 ± 0.3 | 12.3 ± 0.1 |
| 20:0 | 0.7 ± 0.05 |