Table I.
ROS production in embryonic axes during imbibition
H2O2 and superoxide (O2·−) production in embryonic axes isolated from dormant (d) and after-ripened, nondormant (nd) sunflower embryos before imbibition (dry) and from embryos that were imbibed on water for 24 h at 10°C (H2O), or treated for 3 h with 0.1 mm MV (MV) or with gaseous cyanide (HCN) and then transferred for 21 h on water at 10°C. Values are means of five replicates ± sd. DW, Dry weight.
| Treatment | Duration of Imbibition | H2O2
|
O2.−
|
||
|---|---|---|---|---|---|
| d | nd | d | nd | ||
| h | nmol g DW−1 | nmol g DW−1 | |||
| No | 0 (dry) | 1.51 ± 0.21 | 2.10 ± 0.04 | 0.62 ± 0.03 | 0.91 ± 0.06 |
| H2O | 3 | 2.12 ± 0.13 | 2.96 ± 0.09 | 0.63 ± 0.01 | 0.98 ± 0.05 |
| HCN | 3 | 2.81 ± 0.12 | 4.22 ± 0.2 | 0.70 ± 0.06 | 1.01 ± 0.06 |
| MV | 3 | 2.73 ± 0.11 | 3.11 ± 0.15 | 0.70 ± 0.04 | 0.99 ± 0.06 |
| H2O | 24 | 2.50 ± 0.19 | 3.26 ± 0.17 | 0.61 ± 0.05 | 1.10 ± 0.06 |
| HCN | 24 | 4.25 ± 0.35 | 5.05 ± 0.11 | 0.67 ± 0.05 | 1.28 ± 0.06 |
| MV | 24 | 4.51 ± 0.17 | 5.44 ± 0.22 | 0.69 ± 0.03 | 1.20 ± 0.02 |