Table II.
Effect of ROS scavengers on germination of embryos treated by HCN
HCN treatment was carried out for 3 h before transferring the embryos to wet cotton wool at 10°C. ROS scavengers were applied continuously and their concentrations were 1 mm. Data are means of three replicates ± sd. SB, Sodium benzoate; ASA, ascorbic acid.
| Treatment | Germination (%) after 7 d at 10°C of:
|
|
|---|---|---|
| Dormant Embryos | Nondormant Embryos | |
| Water | 18 ± 4 | 96 ± 4 |
| HCN | 96 ± 3 | 97 ± 4 |
| Tiron | 13 ± 5 | 100 ± 0 |
| HCN + Tiron | 24 ± 2 | – |
| SB | 10 ± 3 | 98 ± 1 |
| HCN + SB | 27 ± 3 | – |
| DMTU | 17 ± 2 | 99 ± 1 |
| HCN + DMTU | 35 ± 4 | – |
| ASA | 11 ± 3 | 100 ± 0 |
| HCN + ASA | 28 ± 2 | – |
| GSH | 10 ± 4 | 100 ± 0 |
| HCN + GSH | 30 ± 3 | – |