Figure 3.

Embryo development in wild-type and gsl8 mutant plants. Seeds were harvested from wild-type (A–D) and gsl8-4 homozygous mutant (E–I) plants at different developmental stages and then cleared and viewed using Nomarski optics. Abnormal phenotypes are detectable at the globular (F) and heart (H) stages as well as in mature embryos (I and J). The other five gsl8 mutant alleles showed similar phenotypes to those of gsl8-4. Embryos in E and I were cleared from the same gsl8-4 heterozygous siliques. Embryos in H and J were dissected away from surrounding seed tissues. Bars = 50 μm in A to D, F, and G and 100 μm in E and H to J.