Figure 1.

Mutation analysis of ABRE and RY elements in the SDH2-3 promoter. A, Structure of the mutant constructs. Motifs targeted by in vitro mutagenesis are boxed. Numbers are in relation to the transcription initiation site, indicated by a curved arrow. The sequence of the wild-type promoter is shown (Non mutated), and the mutated nucleotides in each construct are indicated below. B, GUS activity was measured in duplicate seed extracts from 12 to 20 independent transgenic lines carrying the wild-type or mutant promoters fused to GUS. Each point represents one transgenic line, and sd values have been omitted for clarity.