Table I.
Sequence analysis of the Ve locus in various tomato genotypes
Polymorphisms in the CDS of Ve1 and Ve2 isolated from the tomato isogenic lines Craigella GCR26 (ve/ve) and Craigella GCR218 (Ve/Ve) and the cultivars MoneyMaker (ve/ve), Motelle (Ve/Ve), and VFN8 (Ve/Ve).
|
Ve1 Allelesc
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Resistant Genotypes
|
Susceptible Genotypes
|
|||||||||
| SNP Positiona | Nucleotide Sequenceb | Mutation | Protein Mutation | VFN8 Genomic AF272367 (Kawchuk et al., 2001) | Craigella cDNA AF272366 (Kawchuk et al., 2001) | Craigella GCR218 (This Study) | Motelle (This Study) | VFN8 (This Study) | Craigella GCR26 (This Study) | MoneyMaker (This Study) |
| 29/35 | CCTATGGTT | CTATGGCTT | PMV>LWL | − | − | + | + | + | + | + |
| 246 | GTG | GTC | Silent | − | + | − | − | − | + | + |
| 380 | GAC | GCC | D>A | − | − | + | + | + | − | − |
| 610 | ACT | TCT | T>S | − | + | − | − | − | + | + |
| 706 | TCT | ACT | S>T | − | + | − | − | − | + | + |
| 1,220 | TCAGAG | TAGAG | SE>Stop | − | − | − | − | − | + | + |
| 1,548 | AAC | AAG | N>Kd | − | + | − | − | − | + | + |
| 1,888 | GAC | AAC | D>Nd | − | + | − | − | − | + | + |
|
Ve2 Allelesc
|
||||||||||
| Resistant Genotypes
|
Susceptible Genotypes
|
|||||||||
| SNP Positiona | Nucleotide Sequencee | Mutation | Protein Mutation | VFN8 Genomic AF365929 (Kawchuk et al., 2001) | Craigella cDNA AF365930 (Kawchuk et al., 2001) | Craigella GCR218 (This Study) | Motelle (This Study) | VFN8 (This Study) | Craigella GCR26 (This Study) | MoneyMaker (This Study) |
| 1,811 | GTA | GCA | V>A | − | − | + | + | + | − | − |
| 2,761 | GAC | AAC | D>N | − | − | − | − | − | − | + |
| 2,771 | AGA | ACA | R>T | − | − | + | + | + | − | − |
| 2,893 | TCA | CCA | S>P | − | − | + | + | + | − | − |
| 2,934 | CTC | CTT | Silent | − | − | + | + | + | − | − |
| 3,243 | GGT | GGG | Silent | − | − | + | + | + | − | − |
| 3,380/3,383 | TTTTTT | TCTTCT | FF>SS | − | − | + | + | + | + | + |