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In situ localization of β-amylase BAM1 transcripts (A and B) and distribution of starch granules (C and D) in developing caryopses at 12 DAF (A and C) and 16 DAF (B and D). The BAM1 mRNA was detected (indicated by red arrows) in transfer cell layer (A) and in aleurone/subaleurone (A and B). These tissues contain only small or no starch granules (C and D). al, Aleurone; es, endosperm; np, nucellar projection; pe, pericarp; tc, transfer cells. Bars = 250 μm.
