Table I.

Effects of pollination on PsCPS1, PsGA20ox1, PsGA20ox2, PsGA2ox1, PsGA2ox2, PsGA3ox1, and PsGA3ox2 mRNA levels in pea pericarps

Quantitation for PsCPS1, PsGA20ox, and PsGA2ox genes was performed using an Applied Biosystems 7700 sequence detector, and that for the PsGA3ox genes was performed using an Applied Biosystems StepOnePlus sequence detector with 200 ng of total RNA per sample. Due to different PCR efficiencies between the two models, the abundance of PsCPS1, PsGA20ox, and PsGA2ox genes cannot be directly compared with that of the PsGA3ox genes. Data are means ± se (n = 2–3 for pollinated pericarps; n = 2 for unpollinated pericarps).

Pollination Statusa	−2 DAA	−1 DAA		0 DAA		1 DAA		2 DAA		3 DAA
	−	+	−	+	−	+	−	+	−	+	−
PsCPS1	28,135 ± 734	–b	35,432 ± 7,261	15,777 ± 846	13,927 ± 1,691	9,554 ± 516	12,618 ± 196	21,166 ± 2,064	9,259 ± 1,091	17,561 ± 2,197	12,647 ± 2,345
PsGA20ox1	191,230 ± 12,324	–	246,859 ± 20,923	166,630 ± 12,106	218,289 ± 27,713	124,093 ± 1,272	266,480 ± 58,598	71,596 ± 7,185	246,111 ± 13,546	72,797 ± 83	87,527 ± 48,690
PsGA20ox2	5,495 ± 1,942	–	4,239 ± 467	951 ± 373	1,997 ± 712	12 ± 5	410 ± 24	3 ± 1	869 ± 62	4.8 ± 1	143 ± 82
PsGA2ox1	1,016 ± 462	–	60,522 ± 7,091	14,990 ± 2,878	51,045 ± 18,378	4,899 ± 438	14,164 ± 666	1,803 ± 559	13,092 ± 2,701	887 ± 116	21,058 ± 5,898
PsGA2ox2	330 ± 105	–	347 ± 134	39 ± 3	126 ± 44	41 ± 8	79 ± 7	254 ± 15	214 ± 138	449 ± 197	644 ± 94
PsGA3ox1	28 ± 6	–	270 ± 50	2,542 ± 850	878 ± 21	402 ± 36	704 ± 142	156 ± 30	914 ± 199	465 ± 25	3,884 ± 177
PsGA3ox2	0		0	0	0	0	0	0	0	0	0

^aPollination status: +, pericarps from pollinated ovaries; −, unpollinated pericarps emasculated at −2 DAA, which were green and turgid through the equivalent to 3 DAA but did not exceed 10 mm in length.

^b–, Not determined.