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. 2009 May;150(1):448–462. doi: 10.1104/pp.108.132027

Table III.

Accumulation of [14C]GA20, [14C]GA29, and [14C]GA29-catabolite in wild-type SLN and sln mutants after 24 h of incubation with [14C]GA19

Values shown are percentages calculated as (dpm 14C-metabolite after 24 h of incubation)/(dpm [14C]GA19 added to tissue) × 100. Values in parentheses are 14C-metabolite in dpm × 10−3 ± se (n = 3 for wild-type SLN and n = 2 for sln). Amount of [14C]GA19 added per three pods (three pods per extraction) was as follows: all wild-type SLN, 162,525 dpm; SP (sln), 155,954 dpm; 4-Cl-IAA (sln), 111,054 dpm; SPNS (sln), 111,054 dpm.

Treatmenta Pericarp Growthb 14C-Metabolite
[14C]GA20 [14C]GA29 [14C]GA29-Catabolite
mm
SP (wild-type SLN) 18.6 ± 2.3 7.7 ± 1.1 (12.57 ± 1.83) 3.3 ± 1.20 (5.36 ± 1.87) 1.2 ± 0.32 (1.97 ± 0.52)
SPNS (wild-type SLN) 2.0 ± 0.3 1.91 ± 0.14 (3.10 ± 0.31) 0.94 ± 0.23 (1.52 ± 0.36) 1.5 ± 0.59 (2.51 ± 0.96)
4-Cl-IAA (wild-type SLN) 8.0 ± 0.5 5.9 ± 0.76 (11.36 ± 4.23) 14.8 ± 2.50 (24.00 ± 4.05) 4.5 ± 0.43 (7.40 ± 0.70)
SP (sln) 13.6 ± 4.2 13.1 ± 3.9 (16.62 ± 2.21) 1.0 ± 1.00 (1.67 ± 1.67) 0.16 ± 0.16 (0.24 ± 0.24)
SPNS (sln) 0 ± 0 1.19 ± 1.19 (1.32 ± 1.32) 0.30 ± 0.30 (0.33 ± 0.27) ndc
4-Cl-IAA (sln) 11.7 ± 2.0 10.8 ± 2.5 (15.0 ± 5.75) 4.4 ± 1.30 (6.12 ± 2.71) nd
a

Seeds were either left intact (SP) or removed (SPNS) from the pericarp, or seeds were removed and 4-Cl-IAA was applied to the pericarp at 12 h after deseeding.

b

Pericarp growth in length at 48 h after initial pericarp splitting or splitting and seed removal (±se; n = 6–9).

c

nd, Not detected.