Table III.
Accumulation of [14C]GA20, [14C]GA29, and [14C]GA29-catabolite in wild-type SLN and sln mutants after 24 h of incubation with [14C]GA19
Values shown are percentages calculated as (dpm 14C-metabolite after 24 h of incubation)/(dpm [14C]GA19 added to tissue) × 100. Values in parentheses are 14C-metabolite in dpm × 10−3 ± se (n = 3 for wild-type SLN and n = 2 for sln). Amount of [14C]GA19 added per three pods (three pods per extraction) was as follows: all wild-type SLN, 162,525 dpm; SP (sln), 155,954 dpm; 4-Cl-IAA (sln), 111,054 dpm; SPNS (sln), 111,054 dpm.
| Treatmenta | Pericarp Growthb |
14C-Metabolite
|
||
|---|---|---|---|---|
| [14C]GA20 | [14C]GA29 | [14C]GA29-Catabolite | ||
| mm | ||||
| SP (wild-type SLN) | 18.6 ± 2.3 | 7.7 ± 1.1 (12.57 ± 1.83) | 3.3 ± 1.20 (5.36 ± 1.87) | 1.2 ± 0.32 (1.97 ± 0.52) |
| SPNS (wild-type SLN) | 2.0 ± 0.3 | 1.91 ± 0.14 (3.10 ± 0.31) | 0.94 ± 0.23 (1.52 ± 0.36) | 1.5 ± 0.59 (2.51 ± 0.96) |
| 4-Cl-IAA (wild-type SLN) | 8.0 ± 0.5 | 5.9 ± 0.76 (11.36 ± 4.23) | 14.8 ± 2.50 (24.00 ± 4.05) | 4.5 ± 0.43 (7.40 ± 0.70) |
| SP (sln) | 13.6 ± 4.2 | 13.1 ± 3.9 (16.62 ± 2.21) | 1.0 ± 1.00 (1.67 ± 1.67) | 0.16 ± 0.16 (0.24 ± 0.24) |
| SPNS (sln) | 0 ± 0 | 1.19 ± 1.19 (1.32 ± 1.32) | 0.30 ± 0.30 (0.33 ± 0.27) | ndc |
| 4-Cl-IAA (sln) | 11.7 ± 2.0 | 10.8 ± 2.5 (15.0 ± 5.75) | 4.4 ± 1.30 (6.12 ± 2.71) | nd |
Seeds were either left intact (SP) or removed (SPNS) from the pericarp, or seeds were removed and 4-Cl-IAA was applied to the pericarp at 12 h after deseeding.
Pericarp growth in length at 48 h after initial pericarp splitting or splitting and seed removal (±se; n = 6–9).
nd, Not detected.