Figure 2.

Nicotianamine (NA) contents. A, Rosette leaves harvested during the vegetative phase. Plants were grown in a hydroponic system with quarter-strength Hoagland medium and 50 μm Fe under an 11-h light period. Six-week-old plants were used for analysis (Col-0/nas4x-1, n = 8; other mutants, n = 4). FW, Fresh weight; n.d., nondetectable. B, Rosette leaves harvested during the reproductive phase. Plants were grown as in A but under a 16-h light period. Six-week-old plants were used for analysis (n = 8). C, Seeds harvested from plants grown in a hydroponic system as described in B (n = 3).