Metal contents of nas4x-1 mutant and wild-type plants. A, Leaves harvested during the vegetative phase from plants grown under an 11-h light period. B, Leaves harvested during the reproductive phase from plants grown under a 16-h light period. C, Flowers, siliques, and seeds. Plants were grown in a regular hydroponic system. The indicated plant organs were dissected and freeze dried. Metal contents (Fe, Zn, and Cu) were determined by graphite furnace atomic absorption spectroscopy with direct solid sampling. For leaves, n = 8; for flowers/siliques, n = 5; for seeds, n = 6. DW, Dry weight. * P < 0.01 using the t test.