Figure 4.

Gene expression patterns and free Pro levels of transgenic rice plants in response to cold. A, Expression pattern of OsCPT1 in wild-type and OsMYB3R-2 transgenic rice at room temperature (25°C) or low temperature (4°C). Tubulin was used as an internal control. The expression pattern of OsCPT1 to cold stress was confirmed with two independent experiments. B, Cellular free Pro level. Data represent means and se of experiments performed in triplicate. Error bars show se and are from three independent replications of the same experiment. The Pro content determination was confirmed by experiments that were repeated twice. AL1, AL4, and AL5, OsMYB3R-2-antisense lines; FW, fresh weight of materials; OL3, OL5, and OL7, OsMYB3R-2-overexpressing lines; RT, room temperature (25°C); WT, wild type.