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Purification of BD oxidase activity from Arabidopsis siliques
| Chromatographic Step | Protein | Total Activity | Specific Activity | Purification |
|---|---|---|---|---|
| mg | nkat | nkat mg−1 | fold | |
| DEAE-cellulose (DE53) | 300 | 40 | 0.13 | 1 |
| HiTrap-Q HP | 69 | 110 | 1.59 | 12.2 |
| Superdex 200 10/300 GL | 23 | 150 | 6.52 | 50.1 |
| Mono-Q HR 10/10 | 2 | 580 | 290 | 2,230 |
