Whole gland and epithelial fragment transplantation into
Rag1-/- hosts. The genotype of the epithelial fragment
donor is shown above each column, and the magnification values of these
inverted fluorescence images to the left. The boxed regions in A,B,E,F are
shown at higher magnification in C,D,G,H. (A,C) Wild-type
epithelial fragment showing complete filling of the available fat pad and
normal duct morphology (A), with blunt or rounded duct termini (C).
(B,D) Homozygous Ptch1mes gland showing
complete fat pad filling but modestly altered duct morphology (B), with
splayed duct termini (D). (E,G) Wild-type gland showing complete
filling of available fat pad and normal duct morphology (E), with normal blunt
or rounded duct termini (G). (F,H) Homozygous
Ptch1mes fragment showing complete fat pad filling but
modestly altered duct morphology (F), with excessively rounded duct termini
(H). Arrows indicate aberrant termini. Scale bars: 1 mm.