Figure 3.
The ubiquitin-proteasome pathway. A target protein degraded by the ubiquitin-proteasome pathway is first covalently modified by multiple ubiquitin (Ub) molecules in a three-stepped, highly regulated enzymatic process involving an Ub-activating (E1), Ub-conjugating (E2), and the Ub-ligating (E3) enzymes. The ubiquitinated protein is then escorted to the 26S proteasome, recognized by the 19S cap, de-ubiquitinated and then degraded by the catalytic 20S core into oligopeptides. The ubiquitin molecules are released and recycled.