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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Mol Cell Cardiol. 2008 Sep 27;46(1):108–115. doi: 10.1016/j.yjmcc.2008.09.126

Figure 4.

Figure 4

Frictional loading assays measuring the isometric force of the WT (dotted line), NTg (solid line), N47K (short-dashed line), and R58Q (long-dashed line) myosins. The amount of alpha-actinin needed to stop filament sliding velocity gives a measurement of the myosin isometric force. The average actin filament sliding velocity and standard errors in the mean sliding velocity are plotted as a function of alpha-actinin added to the motility assay surface. Data was fit as described in the methods. Both R58Q and N47K showed significant reductions in isometric force compared to the WT myosin.