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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: Biotechnol Bioeng. 2009 Mar 1;102(4):1025–1032. doi: 10.1002/bit.22147

Table II.

Effect of additives on the conformational flexibility of subtilisin Carlsberg in dioxane.

Lyophilized Crown ether colyophilized PEGylated
[NEP] 0.34 ± 0.01 0.34 ± 0.02 0.35 ± 0.01
[VFEP] 0.23 ± 0.04 0.26 ± 0.02 0.26 ± 0.02
akVFEP 0.16 ± 0.07 0.38 ± 0.07 0.55 ± 0.14
[FEP] 0.19 ± 0.04 0.22 ± 0.01 0.18 ± 0.03
a,bkFEP 5.0 ± 0.3 1.1 ± 0.2 2.0 ± 0.8
[SEP] 0.24 ± 0.02 0.20 ± 0.02 0.21 ± 0.03
a,ckSEP 10 ± 1 1.6 ± 0.1 10 ± 1
a

In hours−1.

Values to be multiplied by 10−3.

Values to be multiplied by 10−5.

To convert these values to the actual total number of exchanged hydrogens using the relationship discussed in the Data Analysis Section for instance, Total [NEP]Lyo Dioxane = [(1−0.34) × 434] = 286.4 hydrogens. Likewise, kVFEP for the lyophilized enzyme in dioxane = 433 H × h−1.