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. 2009 Apr 15;23(8):939–950. doi: 10.1101/gad.524609

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Copyright © 2009 by Cold Spring Harbor Laboratory Press

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Figure 5. — Inheritance of DNA methylation patterns at the Copia45 (AT4G37705) locus. (A) Hybridization signals of methylation profiling at Copia45 region for wild type, met1-3, epi01, epi12, and epi28. The parental assignment of informative tiles is provided below each signal graph of epiRILs (color-coded as in Fig. 4A; purple hybridization signal intensity). (B) HpaII restriction map of the Copia45 locus. The diagnostic HpaII site is marked by an asterisk in A and B. (C) Validation of the Copia45 methylation profiles by Southern blot analyses of F₈ generation using HpaII-digested DNA isolated from 10 siblings per sample. (D) Southern blot revealing variation of DNA methylation patterns among F₈ siblings. (E) Inheritance of DNA methylation patterns to F₉ progeny. Four to five progeny (marked 1 to 5) were analyzed for each F₈ progenitor plant (marked above the blot).