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Activation of CACTA transposition in epiRILs revealed by Southern blot analyses of EcoRV-digested DNA and probed as reported previously (Miura et al. 2001). (A) The parental lines wild type, second, and fourth generation met1-3 (WT, m2, and m4, respectively) (left panel), and four random epiRILs (right panel). (B) Transgenerational analysis (F3 to F9) of epi07 (see also Supplemental Fig. 10). (C) Stochastic onset of CACTA transposition in epi07 siblings at the F4 generation.
