Figure 6.
The telomerase machinery is recruited to an unrepaired or slowly repaired DSB. (A) Cdc13p is recruited to a DSB in the donorless strain. ChIP analysis of Cdc13p recruitment was conducted in a “donorless” CDC13-13Myc strain as described in Figure 4. (B,C) Cdc13p is recruited to a DSB that is slowly repaired. Cdc13-myc recruitment was studied in the strain with the Ectopic Donor yJK17 (B) and in the SSA strain YMV2 (C) in a manner similar to A. (D) Telomerase is recruited to a DSB. ChIPs for Est2p-13Myc show that it is recruited to the DSB locus in a donorless cell with kinetics that mirror those of Cdc13p. All ChIPs plotted on the same panel were always carried out simultaneously. Error bars represent one standard error of the mean for at least three independent experiments.