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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: J Neuroimmune Pharmacol. 2008 Jul 25;3(4):257–264. doi: 10.1007/s11481-008-9117-3

Fig. 2.

Fig. 2

Effect of aprepitant on HIV-1 infection of human microglia. Seven-day-cultured microglia were treated with or without aprepitant (10-6 M) for 2 h and then infected with CNS-derived HIV-1 strains (JAGO or JRFL) in the presence or absence of aprepitant. Cultures were re-fed with fresh media containing aprepitant every 3-4 days. Culture supernatants were collected for HIV RT activity at day 12 post-infection (a). Total cellular RNA was extracted and subjected to the real-time RT-PCR for SP (b) and CCR5 (c) mRNA expression. The data shown are the mean ± SD of triplicate cultures, representative of two experiments