Fig. 5.

Effect of human serum on anti-HIV-1 activity of aprepitant in macrophages. Seven-day-cultured macrophages were incubated with or without aprepitant (10^-6 M) at different concentrations of autologous human serum or 10% FCS for 2 h. The cells were infected with HIV-1 (Bal) for 2 h at 37°C in the presence or absence of aprepitant. The human serum cytotoxicity effects were measured by the MTS assays on day 4, following the protocol of manufactory (a). HIV-1 RT activity was analyzed at day 12 post-infection (b). The data shown were presented as the mean ± SD of triplicate cultures, representative of three experiments using cells from three different donors (aprepitant vs. control: *p<0.05)