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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: J Biol Chem. 2007 Mar 28;282(22):16042–16053. doi: 10.1074/jbc.M606781200

Fig. 5. TLR2 and TLR4 agonists induce tyrosine phosphorylation of respective TLRs.

Fig. 5

HEK293T cells were transiently transfected with pCDNA3-huCD14, pEFBOS-HA-huMD-2, along with either pCMV-1-Flag-huTLR2 (A,B) or pCDNA3-huTLR4 (C). Cells were recovered for 24 h, followed by stimulation with TLR2 (STF and Pam3Cys, A and B) and TLR4 (LPS, C) agonists. TLR2 and TLR4 were immunoprecipitated from cell extracts with α-Flag and α-TLR4 H80 Ab, respectively, and subjected to immunoblot analysis for total TLR2 (α-Flag Ab) or TLR4 (H80) expression, as well as TLR tyrosine phosphorylation (α-phosphotyrosine Ab PY20). Shown are data of one out of three experiments.